Time-lapse microscopy showing the behavior of genetic circuits I’ve built in bacteria. Please refer to the supplementary information of the papers listed on the Publication page for additional movies.
The Synchronized Lysis Circuit (SLC) in a strain of S. typhimurium. The bacteria are growing in a microfluidic growth chamber and express a green fluorescent protein (GFP) upon reaching a quorum threshold and activating lysis. After each lysis cycle, a few survivors repopulate the colony and repeat the process.
The Synchronized Lysis Circuit (SLC) in a strain of S. typhimurium which exhibits a longer period due to modifications on the activating gene.
The Synchronized Lysis Circuit (SLC) co-expressed with a therapeutic toxin (Hemolysin E) in S. typhimurium. The bacteria are co-cultured with cancer cells in a device with fluidically connected chambers. The bacteria lyse and release the toxin, resulting in cancer cell death.